Methods for identifying compounds that affect rna/rna...

C - Chemistry – Metallurgy – 07 – H

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C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 9/22 (2006.01) C12P 19/34 (2006.01) C12P 21/04 (2006.01) C12Q 1/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2382348

Disclosed herein are nucleic acid sequences and their optimized subfragments which are located in the mRNA untranslated regions of therapeutically-relevant genes. These sequences specifically bind RNA binding proteins (RBPs) and/or regulate the mRNA functionality. Also disclosed are methods of optimizing a subfragment of a parent nucleic acid sequence such that the RBP binding activity or mRNA functionality of the parent nucleic acid sequence is preserved in the optimized subfragment.

L'invention concerne des séquences d'acides nucléiques et leurs sous-fragments optimisés qui sont situés dans les régions non traduites d'ARNm de gènes d'intérêt thérapeutique. Ces séquences se lient spécifiquement à des protéines de liaison à l'ARN (RBP) et/ou régulent la fonctionnalité d'ARNm. L'invention concerne également des procédés permettant d'optimiser un sous-fragment d'une séquence parent d'acides nucléiques, de façon à préserver l'activité de liaison des RBP ou la fonctionnalité d'ARNm de la séquence parent d'acides nucléiques dans le sous-fragment optimisé.

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