C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/09 (2006.01) A01H 3/00 (2006.01) C07K 14/415 (2006.01) C12N 5/04 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2471371
It has never been considered in the art that cultured cells response to gibberellin. To comprehensively and conveniently identify and isolate gibberellin responsive genes, however, cultured cells which are uniform and easy to handle are appropriate. Considering these advantages of cultured cells, attempts are made to develop a method of identifying and isolating gibberellin responsive genes with the use of cultured cells. Cultured cells are pre-cultured in an auxin-free medium. Then, it is examined whether or not changes in gene expression are detected. As a result, it is surprisingly found out that some genes show changes in the expression after the gibberellin treatment.
Aucune étude antérieure n'a porté sur des cellules cultivées sensibles à la gibbérelline. Pour identifier et isoler de manière globale et pratique les gènes sensibles à la gibbérelline, il convient d'utiliser des cellules cultivées uniformes et facile à manipuler. Compte tenu des avantages des cellules cultivées, on a tenté de mettre sur pied un procédé d'identification et d'isolation des gènes sensibles à la gibbérelline qui fait intervenir des cellules cultivées. Les cellules cultivées ont d'abord été pré-cultivées dans un milieu exempt d'auxine. Que des changements de l'expression génique aient été détectés ou non, ces cellules ont toutes été examinées. Le résultat a montré, de façon surprenante, que l'expression de certains gènes subit un changement après le traitement à la gibbéreline.
Day Robert B.
Minami Eiichi
Shibuya Naoto
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
National Institute Of Agrobiological Sciences
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