Methods for identifying inhibitors of methionine...

Details Methods for identifying inhibitors of methionine... Methods for identifying inhibitors of methionine...

C - Chemistry, Metallurgy

07

K

C07K 5/083 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 2387126

Methods are provided for detecting methionine aminopeptidase (MAP) activity and for detecting inhibitors of MAP. The methods utilize a peptide comprising an N-terminal methionine which can be cleaved from the peptide by MAP, and a C- terminal detection moiety which is released by a second peptidase only if the N-terminal methionine has been cleaved from the peptide. When the peptide is combined with MAP and the second peptidase, the detection moiety is released, while the addition of a MAP inhibitor will inhibit the release of the detection moiety. Reaction mixes, peptides, and kits which are useful for practicing the methods are also provided.

L'invention concerne des procédés destinés à déceler l'activité de la méthionine aminopeptidase (MAP) et à détecter des inhibiteurs de la MAP. Ces procédés font intervenir un peptide comprenant une méthionine à N-terminaison susceptible d'être coupée du peptide par la MAP et une fraction de détection à C-terminaison libérée par une seconde peptidase à condition que la méthionine à N-terminaison ait été coupée du peptide. Lorsque le peptide est combiné à MAP et à la seconde peptidase, la fraction de détection est libérée. L'addition d'un inhibiteur de MAP a pour but d'inhiber la libération de ladite fraction. Des mélanges réactionnels, des peptides et des kits utilisés pour mettre en oeuvre ces procédés font aussi l'objet de cette invention.

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