Methods for identifying, isolating, and utilizing endocrine...

Details Methods for identifying, isolating, and utilizing endocrine... Methods for identifying, isolating, and utilizing endocrine...

C - Chemistry, Metallurgy

12

N

C12N 5/071 (2010.01) A61K 35/39 (2006.01) A61P 5/48 (2006.01) C12M 3/00 (2006.01) C12N 5/00 (2006.01) C12Q 1/04 (2006.01) G01N 33/567 (2006.01) C07K 14/705 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2668223

The presence of the cell surface marker CD 133 or the presence of a glycosylated form of the prominin- 1 gene product on adult pancreatic cells is used to identify pancreatic endocrine progenitor cells, and useful in methods of isolation and enrichment. Isolated pancreatic endocrine progenitor cells can be used for cell based therapy for insulin-dependent diabetes and pancreatectomy patients.

Selon l'invention, la présence d'un marqueur de la surface des cellules, le CD 133, ou la présence d'une forme glycosylée du produit du gène prominine-1 sur des cellules pancréatiques adultes est utilisée pour identifier des cellules progénitrices endocrines pancréatiques et elle est utilisable dans des procédés d'isolement et d'enrichissement. Les cellules progénitrices endocrines pancréatiques isolées peuvent être utilisées pour un traitement à base de cellules du diabète insulino-dépendant et des patients ayant eu une pancréatectomie.

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