C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12P 21/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)
Patent
CA 2289691
The present invention relates to methods for identifying nucleic acid molecules encoding (poly)peptides that interact with target molecules. The method of the present invention is particularly characterized by an in vitro translation step under conditions that allow formation of polysomes in the presence of antisense oligonucleotides complementary to the tag-coding sequence of ssrA-RNA. The present invention further relates to kits that are useful for carrying out the method of the invention.
La présente invention concerne des procédés d'identification de molécules d'acide nucléique codant pour des (poly)peptides interagissant avec des molécules cibles. Le procédé de l'invention est notamment caractérisé par une étape de traduction in vitro, dans des conditions permettant la formation de polysomes en présence d'oligonucléotides antisens complémentaires de la séquence de codage par marqueur de ssrA-ARN. La présente invention concerne en outre des trousses utiles pour l'exécution du procédé de l'invention.
Hanes Jozef
Jermutus Lutz
Pluckthun Andreas
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Pluckthun Andreas
Universitat Zurich
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