C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/67 (2006.01) C07K 14/245 (2006.01) C07K 16/00 (2006.01) C12N 15/10 (2006.01) C12N 15/13 (2006.01) C12N 15/31 (2006.01) C12N 15/62 (2006.01) C12N 15/70 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2305631
The present invention relates to a method for obtaining nucleic acid sequence encoding (poly)peptides which increase the expression yields of periplasmic proteins in functional form upon co-expression of said (poly)peptides and said periplasmic proteins. The invention also provides a method for the identification of said (poly)peptides. Furthermore, the present invention relates to a method for increasing the expression yields of periplasmic proteins in functional form by co-expressing (poly)peptides, for example Skp, FkpA, or a homolog of Skp or FkpA, in bacteria.
La présente invention concerne un procédé permettant d'obtenir des séquences nucléotidiques codant pour des polypeptides qui augmentent les rendements d'expression des protéines périplasmiques fonctionnelles par la coexpression desdits polypeptides et desdites protéines périplasmiques. L'invention se rapporte également à un procédé d'identification des polypeptides, ainsi qu'à un procédé permettant d'augmenter les rendements d'expression des protéines périplasmiques fonctionnelles par coexpression des polypeptides, par exemple de Skp, de FkpA, ou d'un homologue de Skp ou de FkpA dans des bactéries.
Bothmann Hendrick
Pluckthun Andreas
Mccarthy Tetrault Llp
Pluckthun Andreas
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