C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/53 (2006.01) C12N 15/62 (2006.01) C12Q 1/02 (2006.01) C12Q 1/66 (2006.01)
Patent
CA 2321931
A method is provided for identifying polycationic, peptide-like compounds characterized by antibacterial activity against gram negative bacteria. The method takes advantage of the induction of hyperosomotic stress without leakage of cytoplasmic content. The method involves determining whether the compound has the ability to produce phospholipid exchange betwen the lipid bilayers in conjunction with the induction of the osmotic stress response in the target cell. Determining the induction of the osmotic stress response is effected using a bioluminescent detector organism having a osmY-LUX gene fusion.
Cette invention a trait à une méthode d'identification de composés polycationiques, de type peptidique, se caractérisant par leur action antibactérienne à l'encontre de bactéries Gram négatives. Cette méthode tire parti de l'induction d'une contrainte hyperosmotique sans perte de contenu cytoplasmique. Il est déterminé, dans le cadre de cette méthode si le composé est apte à assurer une échange phospholipidique entre les bi-couches lipidiques concurremment avec l'induction de la réponse de contrainte osmotique dans la cellule cible. La détermination de l'induction de la réponse de contrainte osmotique se fait à l'aide d'un organisme détecteur bioluminescent possédant une fusion de gènes osmY-LUX.
Cajal Yolanda
Jain Mahendra Kumar
Van Dyk Tina K.
Bennett Jones Llp
E.i. Du Pont de Nemours And Company
University Of Delaware
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