Methods for identifying signalling molecules

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07K 14/415 (2006.01)

Patent

CA 2400390

The present invention provides methods for identifying and isolating signalling molecules, such as signalling molecules that interact with a plant celled membrane receptor molecule. In the practice of the present invention, the ability of a sample of biological material to induce a pH change in a liquid plant cell culture is used as an assay for the presence of one or more signalling molecules in the biological material.

La présente invention concerne des méthodes permettant d'identifier et d'isoler des molécules de signalisation, notamment les molécules de signalisation qui interagissent avec une molécule de récepteur membranaire des cellules végétales. Dans la pratique de la présente invention, on utilise l'aptitude d'un échantillon de matériel biologique à induire un changement de pH dans une culture liquide de cellules végétales comme méthode d'analyse de la présence d'une ou de plusieurs molécules de signalisation dans ledit matériel biologique.

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