Methods for identifying small molecules that bind specific...

Details Methods for identifying small molecules that bind specific... Methods for identifying small molecules that bind specific...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/02 (2006.01) G01N 27/26 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2443711

The present invention relates to a method for screening and identifying test compounds that bind to a preselected target ribonucleic acid ("RNA"). Direct, non-competitive binding assays are advantageously used to screen libraries of compounds for those that selectively bind to a preselected target RNA. Binding of target RNA molecules to a particular test compound is detected using any physical method that measures the altered physical property of the target RNA bound to a test compound. The structure of the test compound attached to the labeled RNA is also determined. The methods used will depend, in part, on the nature of the library screened. The methods of the present invention provide a simple, sensitive assay for high-throughput screening of libraries of compounds to identify pharmaceutical leads.

L'invention concerne un procédé permettant le criblage et l'identification de composés qui se lient avec un acide ribonucléique (ARN) cible présélectionné. Des méthodes de mesure de liaison directes, non compétitives, permettent de cribler des banques de composés d'une manière aisée afin d'identifier ceux qui se lient sélectivement avec un ARN cible présélectionné. On détecte la liaison des molécules d'ARN cible avec des composés particuliers examinés par un quelconque procédé physique permettant de mesurer les modifications des caractéristiques physiques de l'ARN cible lié avec un des composés testés. On détermine également la structure du composé fixé sur l'ARN marqué. Les procédés utilisés dépendent en partie de la nature de la banque criblée. Les procédés décrits offrent une méthode simple et sensible permettant un criblage haut débit de banques de composés en vue de l'identification de pistes pharmaceutiques potentielles.

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