Methods for identifying stem cells by detecting fluorescence...

G - Physics – 01 – N

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G01N 33/50 (2006.01)

Patent

CA 2738133

Methods are disclosed for the characterization of the stage of development or pathology of a tissue sample, and for identifying pluripotent metakaryotic stem cells, comprising detecting fluorescence of cells and syncytia in fixed samples treated with a non-fluorescent Schiff s base reagent in the absence of extraneous or exogenously added fluorescent dyes.

La présente invention concerne des procédés de caractérisation de létape du développement ou dune pathologie dun échantillon de tissu, et didentification de cellules souches métacaryotes pluripotentes, qui comprennent la détection de la fluorescence des cellules et du syncytium dans des échantillons fixes traités avec un réactif de type base de Schiff non fluorescent en labsence de colorants fluorescents ajoutés de façon extrinsèque ou exogène.

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