Methods for identifying the toxic/pathologic effect of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) C07K 14/47 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2310621

Methods are disclosed for assessing the toxic or pathologic effects of a selected environmental stimulus or reagent on a mammalian cell by determining on a DNA grid a "fingerprint" hybridization pattern. The fingerprint pattern is characteristic of chemically or structurally diverse stimuli or reagents, which have a common adverse effect on gene transcription. A test compound is screened for a similar toxic effect by comparing its hybridization pattern on a similar grid to the fingerprint.

L'invention porte sur un procédé d'évaluation des effets pathologiques de stimuli ou de réactifs environnementaux sur des cellules de mammifère par identification sur une grille d'ADN d'un motif d'hybridation en forme "d'empreinte digitale". Ledit motif est caractéristique de différents stimuli ou réactifs environnementaux chimiques ou structurels présentant un effet adverse commun sur la transcription des gènes. Différentes substances expérimentales sont testées pour rechercher un effet toxique similaire en comparant leur motif d'hybridation sur une grille similaire avec l'empreinte digitale.

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