Methods for identifying therapeutic agents of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) A61K 45/06 (2006.01)

Patent

CA 2510924

The present invention relates to a method for identifying therapeutic agents for reducing and monitoring the growth, erosion, rupture or stability of an atherosclerotic plaque comprising the analysis of the differential expression of at least two genes coding proteins chosen among among Stearoyl CoA desaturase, Phosphatidic acid phosphate, and Phosphoinositide-specific- phospholipase-B1, eventually in association with the analysis of the differential expression of at least one gene coding a protein choosen in the group comprising Aldose reductase and aldehyde reductase, Sphingomyelinase, Acid ceramidase, Ceramide glucosyl transferase, Sphingosin phosphate liase, Thymosine beta 4, Aldehyde dehydrogenase, ATPase Ca++ binding protein and CD163.

La présente invention se rapporte à un procédé d'identification d'agents thérapeutiques permettant de réduire et de réguler la croissance, l'érosion, la rupture ou la stabilité d'une plaque d'athérosclérose. Ledit procédé consiste à analyser l'expression différentielle d'au moins deux gènes codant des protéines sélectionnées parmi la stéaroyl-CoA désaturase, la phosphatase de l'acide phosphatidique, et la phospholipase phosphoinositide-spécifique B1, et éventuellement à analyser conjointement l'expression différentielle d'au moins un gène codant une protéine sélectionnée dans le groupe constitué de l'aldose réductase et l'aldéhyde réductase, la sphingomyélinase, la céramidase acide, la céramide glucosyltransférase, la sphingosine phosphate lyase, la thymosine .beta.-4, l'aldéhyde déshydrogénase, la protéine de liaison ATPase Ca++ et CD163.

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