Methods for improving antibody production

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/13 (2006.01) C07K 16/28 (2006.01) C07K 16/46 (2006.01) C12N 15/09 (2006.01) C12P 21/08 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2667502

The present invention encompasses manufacturing of antibody variants, such as variant of huC242, or fragments thereof, wherein the variants are manufactured by substituting one or more amino acid residues in a parent antibody. Such substitution(s) is preferably done in a variable region framework sequence of the parent antibody comprising a heavy and a light chain. As a consequence of such substitution(s), variant antibodies or fragments thereof show enhanced antibody synthesis when introduced in a host cell as compared to the parent antibody.

La présente invention concerne la fabrication de variantes d'anticorps, comme la variante d'huC242 ou des fragments de celle-ci. Dans ladite invention, les variantes sont fabriquées en remplaçant un ou plusieurs résidu(s) d'acides aminés dans un anticorps parent. Ce ou ces remplacement(s) sont de préférence effectués dans une séquence de squelette à partie variable de l'anticorps parent comprenant une chaîne lourde et une chaîne légère. Suite à ce(s) remplacement(s), les variantes d'anticorps ou leurs fragments présentent une synthèse d'anticorps améliorée par rapport à l'anticorps parent, lors de leur introduction dans une cellule hôte.

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