C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 1/22 (2006.01) B01L 3/00 (2006.01) B05D 5/08 (2006.01) C08J 7/04 (2006.01) C09K 3/18 (2006.01) C12M 1/00 (2006.01) C12M 3/00 (2006.01) C12N 1/00 (2006.01) C12N 5/00 (2006.01) C12Q 1/00 (2006.01) C12Q 1/04 (2006.01) G01N 1/30 (2006.01) G01N 33/566 (2006.01) G01N 33/58 (2006.01) G01N 15/10 (2006.01)
Patent
CA 2675495
The present application is directed to methods of improving cell culture vessel assays. In one aspect the application is directed to a method of reducing the curvature of the meniscus comprising applying a coating material to the interior wall of the vessel, wherein the coating material provides a receding contact angle of about 90 degrees with aqueous solutions and culture media. In another aspect, the application is directed to a method of labeling cells in a first solution by generating droplets of a second solution containing at least one cell-labelling agent and allowing the droplets of the second solution to contact the surface of the first solution.
La présente invention concerne des procédés permettant d'améliorer des dosages en cuve de culture cellulaire. Dans un aspect, l'invention concerne un procédé permettant de réduire la courbure du ménisque qui consiste à appliquer un matériau de revêtement sur la paroi intérieure de la cuve, ce matériau de revêtement permettant d'obtenir un angle de mouillage sortant d'environ 90 degrés avec des solutions aqueuses et un milieu de culture. Dans un autre aspect, l'invention concerne un procédé de marquage de cellules dans une première solution par génération de gouttelettes d'une seconde solution contenant au moins un agent marqueur de cellule et permettant à ces gouttelettes de la seconde solution d'entrer en contact avec la surface de la première solution.
Dossantos Gary
Dowd Jason
Egeler Oliver
Woodside Steven
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Stemcell Technologies Inc.
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