Methods for improving the sequence fidelity of synthetic...

Details Methods for improving the sequence fidelity of synthetic... Methods for improving the sequence fidelity of synthetic...

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) B01D 15/08 (2006.01) C07H 1/06 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2410440

Synthetic oligonucleotides, such as synthetic DNA, often contain sequence errors due to synthetic failures (e.g., side products and/or truncated products). Methods are provided herein for improving the sequence fidelity of synthetic double-stranded oligonucleotides by separative depletion of synthetic failures. Separation is effected by utilization of methodologies in a preparative mode under denaturing conditions. A preferred use of the methods relates to gene synthesis.

L'invention concerne les oligonucléotides synthétiques, par exemple un ADN synthétique, contenant souvent des erreurs de séquence dues à des défaillances de synthèse (produits secondaires et/ou produits tronqués par exemple). L'invention concerne également des méthodes visant à améliorer la fidélité séquentielle des oligonucléotides synthétiques bicaténaires par déplétion de séparation des défaillances de synthèse. La séparation s'effectue selon ces méthodes en mode de préparation dans des conditions de dénaturation. Une utilisation préférée de ces méthodes est associée à la synthèse des gènes.

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