Methods for inducing differentiation of embryonic stem cells...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/02 (2006.01) A01K 67/027 (2006.01) A61K 35/14 (2006.01) A61P 25/28 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01) C12Q 1/02 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2492601

The present invention provides a method for inducing differentiation of an embryonic stem cell into a differentiated neural cell. The present invention further provides a method for producing differentiated neural cells, and a population of cells comprising the differentiated neural cells. Additionally, the present invention provides a method for repopulating a spinal cord in a subject, and a method for treating nervous tissue degeneration in a subject in need of treatment. The present invention further provides neural progenitor cells, differentiated neural cells, and uses of same. Also provided is a transgenic non-human animal containing the differentiated neural cells. The present invention is further directed to a method for isolating a population of differentiated neural cells. Finally, the present invention provides a method for identifying an agent for use in treating a condition associated with neuron degeneration.

La pr~sente invention concerne un proc~d~ pour induire la diff~renciation d'une cellule embryonnaire en cellule neuronale diff~renci~e. L'invention concerne ~galement un proc~d~ de production de cellules neuronales diff~renci~es, ainsi qu'une population de cellules comprenant ces cellules neuronales diff~renci~es. L'invention concerne en outre un proc~d~ pour repeupler la moelle ~pini­re chez un sujet, ainsi qu'une m~thode de traitement d'une d~g~n~rescence des tissus nerveux chez un sujet n~cessitant un traitement. La pr~sente invention porte par ailleurs sur des cellules prog~nitrices neuronales, des cellules neuronales diff~renci~es, ainsi que des utilisations de celles-ci. Un animal non humain transg~nique comprenant lesdites cellules neuronales diff~renci~es est ~galement d~crit. L'invention se rapporte en outre ~ un proc~d~ pour isoler une population de cellules neuronales diff~renci~es. L'invention concerne enfin un proc~d~ pour identifier un agent destin~ ~ Útre utilis~ pour traiter un ~tat associ~ ~ une d~g~n~rescence neuronale.

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