Methods for inducing the differentiation of blood monocytes...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/0784 (2010.01) C12N 5/0783 (2010.01) A61M 1/36 (2006.01) C12M 3/00 (2006.01) A61K 35/14 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2573018

Methods are provided for inducing differentiation of blood monocytes into functional antigen presenting dendritic cells. The blood monocytes are treated by subjecting the monocytes to physical perturbation. The monocytes may be treated, for example, by mechanical or manual shaking in an appropriate container, or the monocytes may be subjected to physical perturbation as they flow through a packed column. The treated monocytes may be co-incubated with disease effector agents, which may be phagocytized by the immature dendritic cells.

L'invention concerne des méthodes d'induction de la différenciation de monocytes sanguins dans un antigène fonctionnel présentant des cellules dendritiques. Les monocytes sanguins sont traités par perturbation physique. Ils peuvent être traités, par exemple, par secouement mécanique ou manuel dans un contenant approprié ou ils peuvent être soumis à une perturbation physique, à mesure qu'ils s'écoulent à travers une colonne remplie. Les monocytes traités peuvent être co-incubés avec des agents effecteurs de maladie qui peuvent être phagocytés par les cellules dendritiques immatures.

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