Methods for inducing the differentiation of blood monocytes...

A - Human Necessities – 61 – K

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A61K 35/00 (2006.01) A61K 35/14 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2717038

Methods are provided for inducing differentiation of blood monocytes into functional antigen presenting dendritic cells. The blood monocytes are treated by exposing the monocytes to proteins adhered to the surface of a treatment device to induce differentiation of the monocytes in to dendritic cells. The treated monocytes may be co-incubated with disease effector agents which have been rendered apoptotic or other source of antigens. The apoptotic disease effector cells are phagocytized by the maturing dendritic cells, hi a preferred embodiment, the blood monocytes are treated in the plastic device without use of a photoactivatable agent and light.

Linvention concerne des procédés dinduction de différentiation de monocytes sanguins dans des cellules dendritiques présentant un antigène fonctionnel. Les monocytes sanguins sont traités par exposition des monocytes à des protéines qui adhèrent à la surface dun dispositif de traitement pour induire une différentiation des monocytes dans les cellules dendritiques. Les monocytes traités peuvent être co-incubés avec des agents effecteurs de maladie qui ont été rendus apoptotiques ou une autre source dantigènes. Les cellules effectrices de maladie apoptotiques sont phagocytées par les cellules dendritiques en voie de maturation. Dans un mode de réalisation préféré, les monocytes sanguins sont traités dans le dispositif en plastique sans utiliser dagent photoactivable ni de lumière.

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