Methods for isolating centromere dna

Details Methods for isolating centromere dna Methods for isolating centromere dna

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2412609

The invention provides efficient methods for the isolation of centromeres from potentially any organism. The methods represents an advance over the prior art in that costly and labor intensive mapping programs are not required. Using the technique, methylated centromere DNA may be isolated from potentially any centromere in an organism. The technique is amenable to mass screenings employing use of arrays comprising libraries of DNA from a target species.

L'invention concerne des méthodes efficaces d'isolation de centromères à partir d'un organisme. Ces méthodes constituent des avancées par rapport aux antériorités en ce qu'elles ne nécessitent pas de programmes de cartographie coûteux et à prédominance de main-d'oeuvre. L'utilisation de cette technique permet d'isoler de l'ADN centromère méthylé à partir d'un centromère quelconque de l'organisme. Cette technique s'applique au dépistage systématique à l'aide de réseaux de banques d'ADN d'une espèce cible.

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