Methods for isolating, enriching and increasing the...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/00 (2006.01) A61K 9/00 (2006.01) A61K 9/16 (2006.01) A61K 35/39 (2006.01) A61K 48/00 (2006.01) A61M 37/00 (2006.01) C12N 5/06 (2006.01) C12S 3/24 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2179341

The present invention provides a novel culture system for the isolation, enrichment and maintenance of neonatal islet cells in vitro, for use in transplantation in vivo. Cellular compositions produced by the culture method are also provided, including mocroencapsulated cells useful for transplantation, for example in diabetes. Also provided is a method of increasing the maturation rate or proliferation rate of a cell utilizing microencapsulated techniques. The invention also provides a method of treatment of a subject having diabetes utilizing cells produced by the culture method described herein.

Nouveau système de culture pour l'isolement, l'enrichissement et le maintien d'îlots de Langerhans in vitro, en vue de leur utilisation dans les transplantations in vivo. Les compositions cellulaires produites par la méthode sont également fournies, y compris les cellules microencapsulées utiles aux transplantations, par exemple dans les cas de diabète. Également fournie, une méthode permettant d'augmenter les taux de maturation ou de prolifération d'une cellule à l'aide de techniques de microencapsulation. L'invention donne également une méthode de traitement d'un sujet atteint de diabète faisant appel aux îlots produits par la méthode en question.

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