Methods for isolating nucleic acids from biological and...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2580661

Methods of isolating nucleic acids from samples of biological or cellular material are disclosed which use solid phase binding materials and which avoid the use of any lysis solution or coating. The use of the solid phase binding materials unexpectedly allow the nucleic acid content of cells to be freed and captured directly and in one step. the new methods represent a significant simplification over existing methods. Nucleic acids can be captured and released in a form suitable for downstream processing in under five minutes. Preferred solid phase materials for use with the methods and compositions of the invention comprise a quaternary onium nucleic acid binding portion.

L'invention concerne des méthodes servant à isoler des acides nucléiques de spécimens de matériaux biologiques ou cellulaires, ce qui consiste à mettre en application des matériaux de liaison en phase solide et permet d'éviter l'utilisation de toute solution ou tout revêtement lytiques. L'utilisation de ces matériaux de liaison en phase solide permet, de façon avantageuse, de libérer le contenu d'acide nucléique des cellules et de le capturer directement et en une étape. Ces nouvelles méthodes apportent une simplification considérable par rapport aux méthodes existantes. Les acides nucléiques peuvent être capturés et libérés sous une forme adéquate à un traitement en aval en 5 minutes seulement. Les matériaux en phase solide préférés utilisés avec ces méthodes et des compositions associées comprennent une partie de fixation d'acide nucléique d'onium quaternaire.

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