Methods for labeling intracytoplasmic molecules

Details Methods for labeling intracytoplasmic molecules Methods for labeling intracytoplasmic molecules

C - Chemistry, Metallurgy

12

Q

C12Q 1/24 (2006.01) C12N 5/08 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/569 (2006.01) G01N 33/72 (2006.01)

Patent

CA 2212796

The invention features a method of labeling a cell containing an intracytoplasmic target molecule involving (1) permeabilizing the plasma membrane of the cell so that (a) a reagent capable of detectably labeling the intracytoplasmic target molecule can traverse the plasma membrane into the cytoplasm of the cell; and (b) substantially all of the intracytoplasmic target molecule and the DNA of the cell remain in the cell; and (2) contacting the cell with the reagent to label the intracytoplasmic target molecule. The method may further involve detecting the label in the cell, and isolating the cell on the basis of detecting the label in the cell. The invention also includes cells permeabilized using the method of the invention.

L'invention concerne un procédé de marquage d'une cellule contenant une molécule cible intracytoplasmique, ce procédé consistant: (1) à rendre perméable la membrane plastique de la cellule de m anière que (a) un réactif capable de marquer de façon détectable la molécule cible intracytoplasmique puisse traverser la membrane plasmique et pénétrer dans le cytoplasme de la cellule; (b) que sensiblement toute la molécule cible intrac ytoplasmique ainsi que l'ADN de la cellule restent dans la cellule; puis (2) à mettre la cellule en contact avec le réactif pour marquer la molécule cible intracytoplasmique. Ce procédé peut comprendre en outre le s étapes consistant à détecter le marqueur dans la cellule et à isoler celle-ci sur la base de la détection du marqueur dans la cellule. L'invention comprend également des cellules rendues perméables à l'aide du procédé de l'invention.

Affiliated with

Also associated with

Rating

Methods for labeling intracytoplasmic molecules does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for labeling intracytoplasmic molecules, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for labeling intracytoplasmic molecules will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1900742