Methods for large scale protein production in prokaryotes

Details Methods for large scale protein production in prokaryotes Methods for large scale protein production in prokaryotes

C - Chemistry, Metallurgy

12

P

C12P 21/02 (2006.01) C12N 9/72 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2428643

The invention belongs to the field of protein production in prokaryotic cells. The invention relates to methods for the production of recombinant DNA-derived heterologous protein in prokaryotic cells, wherein said heterologous protein is secreted extracellularly as an active and correctly folded protein, and the prokaryotic cell contains and expresses a vector comprising the DNA coding for said heterologous protein operably linked to the DNA coding for the signal peptide OmpA.

L'invention concerne le domaine de la production de protéines dans des cellules procaryotes. Elle concerne des procédés de production de protéine hétérologue dérivée d'ADN de recombinaison dans des cellules procaryotes. Ladite protéine hétérologue est sécrétée de manière extracellulaire en tant que protéine active et correctement pliée et la cellule procaryote contient et exprime un vecteur comprenant l'ADN codant ladite protéine hétérologue fonctionnellement liée à l'ADN codant le peptide signal OmpA.

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