Methods for long-range sequence analysis of nucleic acids

Details Methods for long-range sequence analysis of nucleic acids Methods for long-range sequence analysis of nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12P 19/24 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2580070

Provided are methods for sequencing a target nucleic acid by fragmenting a target nucleic acid, hybridizing fragments to an array of capture oligonucleotides, determining the mass of the hybridized fragments, and constructing a nucleotide sequence of the target nucleic acid from the mass measurements.

L'invention concerne des méthodes de séquençage d'un acide nucléique cible par fragmentation d'un acide nucléique cible, par hybridation des fragments avec un réseau d'oligonucléotides de capture, par détermination de la masse des fragments hybridés, et par construction d'une séquence nucléotidique de l'acide nucléique cible à partir des mesures de masse.

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