C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 19/34 (2006.01) C07H 19/00 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2371890
The invention concerns the use of morpholino-nucleosides of formula (I) wherein: R?1¿ represents a nucleic base and R?2¿ represents a group corresponding to the following formulae: -(CH¿2?)¿n?-NH¿2?, -(CH¿2?)¿n?-SH, -(CH¿2?)¿n?-COOH, -(CH¿2?)¿n?-OH, -(CH¿2?)¿n?-NH-R?3¿, (CH¿2?)¿n?-SR?3¿-(CH¿2?)¿n?-CO-R?3¿, -(CH¿2?)¿n?-OR?3¿ wherein: n is an integer ranging from 1 to 12 and R?3¿ is a group derived from a marker, a protein, an enzyme, a fatty acid or a peptide, as chain terminators in a DNA or RNA sequencing process by Sanger method, or for marking DNA or RNA fragments.
L'invention concerne l'utilisation de morpholino-nucléosides de formule (I): dans laquelle R1 représente une base nucléique et R2 représente un groupe répondant à l'une des formules suivantes: -(CH2)n-NH2, -(CH2)n-SH, -(CH2)n- COOH, -(CH2)n-OH, -(CH2)n-NH-R3, -(CH2)n S-R3, -(CH2)n-CO-R3, -(CH2)n-OR3 dans lesquelles n est un nombre entier allant de 1 à 12 et R3 est un groupe dérivé d'un marqueur, d'une protéine, d'une enzyme, d'un acide gras ou d'un peptide, comme terminateurs de chaînes dans un procédé de séquençage d'ADN ou d'ARN par la méthode de Sanger, ou pour le marquage de fragments d'ADN ou d'ARN.
Issartel Jean-Paul
Marciacq Florence
Molko Didier
Mouret Jean-Francois
Sauvaigo Sylvie
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