Methods for making polynucleotides and purifying...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/62 (2006.01) C07H 1/06 (2006.01) C07H 21/00 (2006.01) C07K 16/46 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/13 (2006.01) C12P 19/34 (2006.01) C12P 21/02 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2473434

The invention provides methods for identifying and purifying double-stranded polynucleotides lacking base pair mismatches, insertion/deletion loops and/or nucleotide gaps. The invention provides libraries of nucleic acid building blocks and methods for generating any nucleic acid sequence, including synthetic genes, antisense constructs and polypeptide coding sequences. The invention provides chimeric antigen binding molecules and the nucleic acids that encode them.

L'invention concerne des procédés d'identification et de purification de polynucléotides double brin sans mésappariements de paires de base, sans boucles d'insertion/délétion et/ou sans brèches de nucléotides. L'invention concerne également des banques d'unités de construction d'acides nucléiques, ainsi que des procédés de production de séquence d'acides nucléiques, notamment de gènes synthétiques, de constructions antisens et de séquences de codage de polypeptides. L'invention concerne encore des molécules de liaison d'antigènes chimériques, ainsi que les acides nucléiques codant lesdits antigènes.

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