Methods for making recombinant cells

Details Methods for making recombinant cells Methods for making recombinant cells

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07K 16/28 (2006.01) C12N 15/65 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2353575

Disclosed are methods for producing recombinant cells and especially recombinant mammalian cell lines with enhanced expression of an amino acid sequence. Also disclosed are recombinant mammalian cell lines producing high levels of the amino acid sequence. The methods and recombinant cell lines of the invention have a number of useful applications including use in the efficient and large-scale production of recombinant proteins and polypeptides.

L'invention concerne des méthodes de fabrication de cellules recombinées et, en particulier, de lignées de cellules recombinées de mammifères, qui présentent une expression améliorée d'une séquence d'acides aminés. L'invention concerne également des lignées de cellules recombinées de mammifères, qui produisent la séquence d'acides aminés en grandes quantités. Les méthodes et les lignées de cellules recombinées de l'invention s'utilisent pour de nombreuses applications, notamment pour la production efficace et intensive de protéines et de polypeptides recombinés.

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