C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/10 (2006.01)
Patent
CA 2344625
The present invention relates generally to methods of labeling, sorting and screening populations of nucleic acids. More particularly, the present invention relates to a method for sorting and comparing complex populations of nucleic acid, such as cDNA libraries. These complex populations of nucleic acid may be derived from cells or tissue types having variations in phenotype of potential clinical interest. The method is referred to generally as the ValiGeneSM <u>P</u>eptide- <u>L</u>abeled <u>O</u>ligonucleotide method, or VG- PLOSM, and involves the use of distinguishable and identifiable peptide tags linked to identical oligonucleotide primers.
L'invention concerne généralement des procédés de marquage, de tri et de criblage de populations d'acides nucléiques. Elle porte notamment sur un procédé de tri et de comparaison de populations complexes d'acides nucléiques, telle que des banques d'ADNc. Ces populations complexes peuvent être dérivées de cellules ou de types de tissus comportant des variations du phénotype présentant un éventuel intérêt clinique. Le procédé est généralement appelé VG-PLO?SM¿, procédé à Oligonucléotides à marquage peptidique, ou et implique l'utilisation d'étiquettes peptidiques identifiables, liées à des amorces olignonucléotidiques identiques.
Iris Francois J.-M.
Pourny Jean-Louis
Osler Hoskin & Harcourt Llp
Valigen (us) Inc.
Valigene Corporation
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