Methods for mediating gene suppresion by using factors that...

Details Methods for mediating gene suppresion by using factors that... Methods for mediating gene suppresion by using factors that...

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2410947

The present invention is concerned with methods for enhancing gene suppression in cells and in particular it is concerned with improved methods for enhancing RNAi-mediated gene silencing by manipulation of factors associated with RNAi. The present invention is also concerned with methods for identifying factors which down-regulate as well as those which up-regulate RNAi. It is also concerned with genetic constructs useful for enhancing or modulating gene silencing and cells harbouring such constructs.

Cette invention, qui a trait à des méthodes permettant d'améliorer la suppression de gène dans des cellules, concerne notamment des améliorations apportées à des techniques permettant d'inhiber un gène à médiation ARNi par manipulation de facteurs associé à l'ARNi. Cette invention concerne également des procédés d'identification de facteurs régulant à la baisse ou à la hausse l'ARNi. Elle concerne des produits de synthèse génétique des plus utiles pour améliorer ou moduler l'inhibition de gène et des cellules comportant ces produits de synthèse.

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