Methods for monitoring eukaryotic cell integrity, growth...

Details Methods for monitoring eukaryotic cell integrity, growth... Methods for monitoring eukaryotic cell integrity, growth...

C - Chemistry, Metallurgy

12

Q

C12Q 1/48 (2006.01) C12Q 1/66 (2006.01)

Patent

CA 2373160

A method for detecting the presence of a lysed eukaryotic cell in a sample, said method comprising: (i) adding adenosine diphosphate (ADP) to said sample under conditions which allows the conversion of ADP to adenosine triphosphate (ATP) by cellular adenylate kinase, (ii) detecting ATP in said sample and relating that to the presence of adenylate kinase and thus to the presence of lysed cells. The method is useful in detecting the cell lysis, for example when screening for drugs which are required to cause lysis, for example for use in tumour therapy. However, in addition, cells may, in a preliminary step, be lysed and the contents quantitated in order to establish for example the health of condition of the cells or to detect the presence of cells in sample such as milk or urine, for diagnostic purposes.

Une méthode permettant de détecter la présence d'une cellule eucaryote lysée dans un échantillon. Cette méthode consiste (i) à ajouter de l'adénosine diphosphate (ADP) audit échantillon dans des conditions permettant la conversion de l'ADP en adénosine triphosphate (ATP) par adenylate kinase cellulaire, (ii) à détecter l'ATP dans cet échantillon et à le mettre en rapport avec la présence d'adenylate kinase et donc avec la présence de cellules lysées. La méthode se révèle efficace dans la détection de lyse cellulaire, par exemple, lors du criblage de médicaments causant la lyse, utile notamment dans le traitement de tumeurs. En outre, les cellules peuvent, dans une étape préliminaire, être lysées et leur contenu quantifié de manière à établir, par exemple, l'état de santé des cellules ou de détecter la présence de cellules dans un échantillon tel que du lait, de l'urine, à des fins de diagnostic.

Affiliated with

Also associated with

Rating

Methods for monitoring eukaryotic cell integrity, growth... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for monitoring eukaryotic cell integrity, growth..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for monitoring eukaryotic cell integrity, growth... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1976489