C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 1/34 (2006.01) C07K 1/16 (2006.01) C07K 1/18 (2006.01) C07K 1/20 (2006.01) C07K 1/22 (2006.01) G01N 33/68 (2006.01) G01N 30/84 (2006.01)
Patent
CA 2465214
The invention provides methods of monitoring the production of a target polypeptide by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken form multiple cell culture batches or of samples taken at different times from a given batch. The invention additionally provides methods for monitoring the purification of a target polypeptide from a mixture by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken at various times during a purification process. In addition, the invention relates to methods of identifying conditions that can be used in increasing the scale of a given purification process.
L'invention concerne des procédés permettant de surveiller la production d'un polypeptide cible par génération de profils d'échantillons spectraux de masse à désorption/ionisation de surface par impact laser améliorée prélevés dans plusieurs lots de culture cellulaire ou d'échantillons prélevés à différents moments dans un lot donné. L'invention concerne en outre des procédés permettant de surveiller la purification d'un polypeptide cible à partir d'un mélange par génération de profils d'échantillons spectraux de masse à désorption/ionisation de surface par impact laser améliorée prélevés à différents moments dans un lot donné lors d'un processus de purification. De plus, l'invention concerne des procédés permettant d'identifier les conditions qui permettent d'accroître l'échelle d'un processus de purification donné.
Boschetti Egisto
Bradbury Lisa
Davies Huw
Lomas Lee
Pham Thang T.
Ciphergen Biosystems Inc.
Smart & Biggar
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