Methods for obtaining cyclopamine

Details Methods for obtaining cyclopamine Methods for obtaining cyclopamine

C - Chemistry, Metallurgy

07

J

C07J 69/00 (2006.01) C07G 5/00 (2006.01) C07J 21/00 (2006.01)

Patent

CA 2636807

The present invention relates to a process for extracting, purifying and isolating cyclopamine from cyclopamine-containing biomass comprising the steps of contacting cyclopamine-containing organic matter with an aqueous extractant capable of extracting cyclopamine, separating the extracted organic matter from the cyclopamine/extract solution, concentrating the cyclopamine/extract solution leaving a concentrated aqueous solution, contacting the concentrated aqueous solution with an organic extractant capable of selectively extracting cyclopamine from the aqueous phase, concentrating the cyclopamine-containing organic phase, providing an enriched extract containing cyclopamine, separating cyclopamine from the enriched extract using column chromatography, providing at least one fraction containing cyclopamine, and crystallizing select chromatography fractions to provide purified cyclopamine.

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