C - Chemistry – Metallurgy – 07 – J
Patent
C - Chemistry, Metallurgy
07
J
C07J 69/00 (2006.01) C07J 21/00 (2006.01) C07J 43/00 (2006.01)
Patent
CA 2727986
The present invention relates to a process for extracting, purifying and isolating cyclopamine from cyclopamine-containing biomass comprising the steps of contacting cyclopamine-containing organic matter with an extractant (e.g., aqueous extractant) capable of extracting cyclopamine, separating the extracted organic matter from the cyclopamine/extract solution, concentrating the cyclopamine/extract solution leaving a concentrated aqueous solution, contacting the concentrated aqueous solution with an organic extractant capable of selectively extracting cyclopamine from the aqueous phase, concentrating the cyclopamine--containing organic phase, providing an enriched extract containing cyclopamine, separating cyclopamine from the enriched extract using column chromatography, providing at least one fraction containing cyclopamine, and crystallizing select chromatography fractions to provide purified cyclopamine.
La présente invention porte sur un procédé pour l'extraction, la purification et l'isolement de cyclopamine à partir de biomasse contenant de la cyclopamine, consistant à mettre en contact de la matière organique contenant de la cyclopamine avec un agent d'extraction (par exemple un agent d'extraction aqueux) apte à extraire la cyclopamine, séparer la matière organique extraite de la solution de cyclopamine/extrait, concentrer la solution de cyclopamine/extrait, ce qui laisse une solution aqueuse concentrée, mettre en contact la solution aqueuse concentrée avec un agent d'extraction organique apte à extraire sélectivement la cyclopamine de la phase aqueuse, concentrer la phase organique contenant la cyclopamine, ce qui fournit un extrait enrichi contenant de la cyclopamine, séparer la cyclopamine de l'extrait enrichi à l'aide d'une chromatographie sur colonne, ce qui fournit au moins une fraction contenant de la cyclopamine, et faire cristalliser les fractions de chromatographie choisies pour fournir de la cyclopamine purifiée.
Kadali Satyasagar
Splinter Steven
Mcfadden Fincham
Radient Technologies Inc.
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