C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/12 (2006.01) C12N 9/52 (2006.01)
Patent
CA 2559415
The present invention provides a method for obtaining thermostable enzymes. The present invention also provides variants of DNA polymerase I from Thermus aquaticus. The present invention further provides methods of identifying mutant DNA polymerases having enhanced catalytic activity. The present invention also provides polynucleotiides, expression systems, and host cells encoding the mutant DNA polymerases. Still further, the present invention provides a method to carry out reverse transcriptase-polymerase chain reaction (RT-PCR) and kits to facilitate the same.
L'invention concerne un procédé d'obtention d'enzymes thermostables. L'invention concerne également des variants d'ADN polymérase I provenant de Thermus aquaticus. L'invention concerne également des procédés d'identification d'ADN polymérases mutantes à activité catalytique améliorée. L'invention concerne également des polynucléotides, des systèmes d'expression, et des cellules hôtes codant pour les ADN polymérases mutantes. L'invention concerne en outre un procédé permettant d'effectuer une amplification en chaîne par polymérase avec transcriptase inverse (RT-PCR) et des kits permettant de faciliter ce procédé.
Ferris Stephane
Jestin Jean-Luc
Vichier-Guerre Sophie
Centre National de La Recherche Scientifique (cnrs)
Institut Pasteur
Robic
LandOfFree
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