Methods for optimizing clinical responsiveness to...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) A61K 31/519 (2006.01) A61P 19/02 (2006.01) A61P 29/00 (2006.01) A61P 35/00 (2006.01) A61P 37/00 (2006.01) C12N 15/12 (2006.01) C12N 15/54 (2006.01) C12Q 1/48 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2539953

The present invention provides methods for optimizing clinical responsiveness to chemotherapy in an individual through genotypic analysis of polymorphisms in at least one gene. The methods of the present invention may further comprise determining the level of at least one long-chain methotrexate polyglutamate (MTXPG) in a sample obtained from the individual. The present invention also provides methods for generating a pharmacogenetic index for predicting clinical responsiveness to chemotherapy in an individual through genotypic analysis of polymorphisms in at least one gene. In addition, the present invention provides methods for optimizing therapeutic efficacy of chemotherapy in an individual by calculating the level of at least one long- chain MTXPG in a sample obtained from the individual.

La présente invention concerne des méthodes permettant d'optimiser la réactivité clinique à une chimiothérapie chez un individu par l'intermédiaire de l'analyse génotypique de polymorphismes dans au moins un gène. Ces méthodes peuvent, en outre, consister à déterminer le niveau d'au moins un polyglutamate de méthotrexate (MTXPG) à chaîne longue dans un échantillon prélevé chez un individu. Cette invention a aussi pour objet des méthodes de génération d'un index pharmacogénétique permettant de prédire une réactivité clinique à la chimiothérapie chez un individu par l'intermédiaire d'une analyse génotypique de polymorphismes dans au moins un gène. En outre, ladite invention a trait à des méthodes d'optimisation de l'efficacité thérapeutique de la chimiothérapie chez un individu par calcul du niveau d'au moins un polyglutamate de méthotrexate (MTXPG) à chaîne longue dans un échantillon prélevé chez un individu.

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