Methods for packaging propagation-defective vesicular...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/113 (2010.01) A61K 39/205 (2006.01) C07K 14/145 (2006.01) C12N 5/10 (2006.01) C12N 7/04 (2006.01) C12N 15/47 (2006.01) C12N 15/63 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2710166

A method of producing propagation-defective Vesicular Stomatitis Virus (VSV) is provided. The method involves providing a cell that includes an optimized VSV G gene, wherein expression of VSV G protein from the optimized VSV G gene is inducible; and inducing the cell to express VSV G protein from the optimized VSV G gene. The method also involves infecting the induced cell with an attenuated VSV; growing the infected cells in culture; and recovering attenuated VSV from the culture.

La présente invention concerne un procédé de production du virus de la stomatite vésiculaire (VSV) à propagation défectueuse. Le procédé comprend les étapes consistant à mettre à disposition une cellule qui comprend un gène G du VSV optimisé, l'expression de la protéine G du VSV à partir du gène G du VSV optimisé pouvant être induite; et à induire l'expression de la protéine G du VSV dans la cellule à partir du gène G du VSV optimisé. Le procédé comprend également les étapes consistant à infecter la cellule où a eu lieu l'induction avec un VSV atténué; à mettre les cellules infectées en culture; et à récupérer les VSV atténués à partir de la culture.

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