Methods for packaging propagation-defective vesicular...

C - Chemistry – Metallurgy – 12 – N

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C12N 7/04 (2006.01) C07K 14/145 (2006.01)

Patent

CA 2710356

A method of producing propagation-defective Vesicular Stomatitis Virus (VSV) in a cell culture is provided. The method involves introducing a plasmid vector encoding an optimized VSV G gene into a cell; expressing VSV G protein from the optimized VSV G gene; and introducing a propagation-defective VSV into the cell expressing the VSV G protein encoded by the optimized VSV G gene. The method further includes growing the cells in culture; and recovering the propagation-defective VSV from the culture.

La présente invention concerne un procédé permettant de produire le virus de la stomatite vésiculaire (VSV) à défaut de propagation dans une culture cellulaire. Le procédé implique l'introduction d'un vecteur plasmidique codant pour un gène VSV G optimisé dans une cellule; l'expression de la protéine VSV G à partir du gène VSV G optimisé; et l'introduction d'un VSV à défaut de propagation dans la cellule exprimant la protéine VSV G codée par le gène VSV G optimisé. Le procédé comprend en outre la mise en culture des cellules et la récupération du VSV à défaut de propagation à partir de la culture.

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