Methods for particle-assisted polynucleotide immunization...

A - Human Necessities – 61 – K

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A61K 48/00 (2006.01) A61K 33/00 (2006.01) A61K 39/00 (2006.01) A61K 39/02 (2006.01) A61K 39/12 (2006.01) A61K 39/39 (2006.01) A61K 41/00 (2006.01) A61N 1/30 (2006.01) A61P 37/04 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2470322

Methods are provided for enhancing an immune response induced by administration of a DNA vaccine. In the invention methods a DNA vaccine encoding an antigen and non-chemically associated adjuvant particles are injected into muscle, dermal or mucosal tissue of a subject at substantially the same time and the tissue is subjected to a pulsed electric field of sufficient strength to result in the DNA vaccine entering cells of the target tissue. The immune response to the antigen is enhanced as compared to when the DNA vaccine is administered alone or in combination with either of the electric pulses or the adjuvant particles without the other.

L'invention concerne des procédés visant à améliorer une réponse immunitaire induite via l'administration d'un vaccin d'immunisation par ADN, au moyen d'une injection intramusculaire, intradermique ou intramuqueuse de ce type de vaccin codant un antigène, d'une part, et de particules d'adjuvant associées non chimiquement, d'autre part, sensiblement au même moment, sachant que le tissu considéré est soumis à un champ électrique pulsé d'intensité suffisante pour faire pénétrer ledit vaccin dans les cellules du tissu cible. On améliore ainsi la réponse immunitaire vis-à-vis de l'antigène, en comparaison avec une administration de vaccin d'immunisation par ADN faite isolément ou bien en combinaison avec les impulsions électriques ou avec les particules d'adjuvant sans les impulsions électriques.

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