Methods for perfusion and plating of primary hepatocytes and...

Details Methods for perfusion and plating of primary hepatocytes and... Methods for perfusion and plating of primary hepatocytes and...

C - Chemistry, Metallurgy

12

N

C12N 5/071 (2010.01) C12Q 1/02 (2006.01)

Patent

CA 2493676

The present invention provides methods for culturing primary hepatocytes with improved long term function and improved viability, by plating the hepatocytes in the presence of an anti-oxidants) as well as an agent(s) which is a functional inhibitor of enzymes that generate reactive oxygen and reactive nitrogen species. One preferred embodiment provides a combination of 2-oxo- thizolidine and tocopherol succinate. Another preferred embodiment provides a combination of NG-methylarginine and mannitol.

L'invention concerne des procédés de culture d'hépatocytes primaires présentant une meilleure fonction à long terme ainsi qu'une meilleure viabilité. Ces procédés consistent à mettre lesdits hépatocytes en culture sur boîte de Pétri, en présence d'au moins un antioxydant ainsi que d'au moins un agent servant d'inhibiteur fonctionnel d'enzymes qui génèrent des espèces d'oxygène réactif et d'azote réactif. Dans un mode de réalisation préféré, cette invention se rapporte à une association de 2-oxo-thizolidine et de tocophérol succinate. Dans un autre mode de réalisation, l'invention concerne une association de N<SP>G</SP>-méthylarginine et de mannitol.

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