Methods for preparing t-cells for cell therapy

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 5/0783 (2010.01) A61K 35/14 (2006.01) A61K 39/00 (2006.01) C12M 3/00 (2006.01) A61K 39/395 (2006.01) C07K 16/28 (2006.01)

Patent

CA 2555714

T-cells are generated with enhanced immunostimulatory capabilities for use in self therapy treatment protocols, by utilizing a biodegradable device with a biodegradable support that has one or more agents that are reactive to T-cell surface moieties. The biodegradable devices are mixed with the T-cells sufficiently so that the one or more agents cross-link with the T-cells~ surface moieties and deliver a signal to the T-cells to enhance immunostimulatory capabilities.

La présente invention se rapporte à des procédés permettant de générer des lymphocytes T présentant des capacités immunostimulatrices améliorées, lesquels sont destinés à des protocoles de traitement dans le cadre de la thérapie cellulaire. Les procédés selon l'invention font appel à un dispositif biodégradable doté d'un support biodégradable contenant un ou plusieurs agents qui sont réactifs aux groupes fonctionnels de surface des lymphocytes T. Lesdits dispositifs biodégradables sont suffisamment mélangés aux lymphocytes T pour que le ou les agents forment des réseaux avec les groupes fonctionnels de surface des lymphocytes T et fournissent un signal auxdits lymphocytes T de façon à améliorer leurs capacités immunostimulatrices.

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