Methods for producing a549 cell lines stable in serum-free...

Details Methods for producing a549 cell lines stable in serum-free... Methods for producing a549 cell lines stable in serum-free...

C - Chemistry, Metallurgy

12

N

C12N 5/071 (2010.01) C12N 7/00 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2551026

The present invention provides methods for adapting cells, such as A549 cells, to growth in serum-free and animal material-free medium suspension culture. The present invention provides methods for preparing viruses, such as adenovirus, from the A549 cells adapted for growth in serum-free and animal material-free medium in suspension culture.

L'invention concerne des méthodes servant à adapter des cellules, telles que des cellules A549, à une croissance dans un milieu de culture en suspension exempt de sérum et de produits animaux. Elle concerne des méthodes servant à préparer des virus, tels que des adénovirus, à partir de ces cellules A549 conçues pour croître dans des milieux de culture en suspension exempts de sérum et de produits animaux.

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