Methods for producing and analyzing biopolymer ladders

G - Physics – 01 – N

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G01N 33/68 (2006.01) C07H 21/00 (2006.01) C07K 1/12 (2006.01) C12P 21/06 (2006.01) C12Q 1/34 (2006.01) C12Q 1/37 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2221727

Methods of producing biopolymer ladders and their use to obtain structural information about the biopolymer. The ladders are produced by settling up catalytic cleavage and terminating reactions at the end of biopolymer molecules. The terminating reactions terminate cleavage of a percentage of the biopolymer molecules at each round of cleavage.

L'invention se rapporte à des procédés de production d'échelles de biopolymères et à leur utilisation permettant d'obtenir des informations structurelles sur le biopolymère. On produit ces échelles en établissant un clivage catalytique et des réactions terminales à l'extrémité des molécules des biopolymères. Les réactions terminales terminent le clivage d'un pourcentage de molécules de biopolymères à chaque série de clivage.

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