Methods for producing and preparing cells for cell therapy

Details Methods for producing and preparing cells for cell therapy Methods for producing and preparing cells for cell therapy

C - Chemistry, Metallurgy

12

N

C12N 5/06 (2006.01) C12N 5/02 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2377906

The invention features methods for expansion and packaging of cells. Expansion is achieved by providing cells that include neural progenitor cells; plating the cells in culture vessels at an average density of 1 X 105 to 7 X 105 cells/cm2; and culturing said cells in culture medium and under conditions permissible for proliferation of said neural progenitor cells, wherein the volume of medium results in an initial cell density of between 5 X 104 and 1.5 X 105 cells per milliliter of medium. Preparation of cells for transplantation includes providing a cell suspension that includes single cells, aggregates of fewer than two hundred cells, or a combination thereof; and re-aggregating the cells in said cell suspension, wherein greater than 50 % of reaggregates consist of between 25 and 500 cells/reaggregate.

L'invention concerne des méthodes d'expansion et de conditionnement de cellules. L'expansion consiste à prendre des cellules contenant des précurseurs neuronaux; à étaler les cellules en boîte de Petri dans des récipients de culture avec une densité moyenne comprise entre 1 X 10?5¿ et 7 X 10?5¿ cellules/cm?2¿ ; puis à mettre en culture ces cellules dans un milieu de culture et dans des conditions favorisant la prolifération des précurseurs neuronaux, le volume du milieu produisant une densité cellulaire initiale comprise entre 5 X 10?4¿ et 1,5 X 10?5¿ cellules par millimètre de milieu. La préparation des cellules en vue d'une transplantation consiste à prendre une suspension cellulaire contenant des cellules individuelles, des agrégats de moins de deux cents cellules, ou une combinaison des deux; puis à constituer de nouveaux agrégats de cellules dans la suspension cellulaire, plus de 50 % des nouveaux agrégats contenant entre 25 et 500 cellules/nouvel agrégat.

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