Methods for producing ceramide using transformed yeast

Details Methods for producing ceramide using transformed yeast Methods for producing ceramide using transformed yeast

C - Chemistry, Metallurgy

12

P

C12P 13/00 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2632534

Disclosed is a process for production of humanized ceramide in an yeast cell. The process comprises the following steps 1) and 2): 1) introducing a sphingoid .DELTA.4-desaturase gene (DES1) into an yeast cell by transformation; and 2) deactivating the expression of an yeast sphinganine C4- hydroxylase gene (SUR2) in the yeast cell by transformation.

La présente invention concerne un procédé pour la production d'un céramide humanisé dans une cellule de levure. Le procédé comprend les étapes 1) et 2) suivantes: 1) l'introduction d'un gène de .DELTA.4-désaturase à base de sphingoïde (DES1) dans la cellule de levure par transformation; et 2) la désactivation de l'expression d'un gène sphinganine C4-hydroxylase (SUR2) dans la cellule de levure par transformation.

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