Methods for producing eg iii cellulase, xylanase or a...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/56 (2006.01) C07K 1/30 (2006.01) C07K 1/36 (2006.01) C12N 9/42 (2006.01) C12N 15/80 (2006.01)

Patent

CA 2133436

Disclosed are methods for producing substantially pure EG III cellulase or xylanase, from Trichoderma spp. In particular, the methods of the present invention comprise separation of the EG III cellulase or xylanase component from an aqueous mixture of enzymes by the addition of polyethylene glycol to the aqueous mixture to create a two-phase system and separating the EG III-rich phase. The present invention is also directed in part to methods for preparing substantially pure EG III cellulase component or xylanase component by fractionation or by a combination of the above two methods.

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