Methods for producing enhanced antigenic enteric bacteria...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/20 (2006.01) A61K 39/02 (2006.01) C07K 16/12 (2006.01) C12Q 1/04 (2006.01) C12Q 1/18 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2202027

Methods using in vitro processes are disclosed for inducing or enhancing expression of enteric bacterial antigens or virulence factors. The methods, therefore, produce antigenically enhanced enteric bacteria. Also methods for using the antigenically enhanced bacteria are also disclosed, as well as vaccines containing the enteric bacteria. Specifically, a whole enteric bacteria or components thereof are provided by Helicobacter species. Also there are other enteric bacteria which are useful for the disclosed invention. One type, Campylobacter jejuni is graphically depicted wherein the results of high-performance liquid chromatography of monosaccharides from surface extract hydrolysates of C. jejuni are shown.

L'invention concerne des méthodes in vitro permettant d'induire ou d'améliorer l'expression d'antigènes bactériens entériques et/ou d'agressines et de produire ainsi des bactéries entériques à action antigène améliorée. L'invention porte également sur des méthodes d'utilisation desdites bactéries entériques à action antigène améliorée et sur des vaccins les contenant. Plus spécifiquement, une bactérie entérique entière ou des composants de celle-ci sont produits par l'espèce Helicobacter. D'autres bactéries entériques sont également utiles pour les procédés selon l'invention. Un type de bactérie, Campilobacter jejuni, est représenté graphiquement, et les résultats de la chromatographie en phase liquide puissante des monosaccharides d'extraits superficiels d'hydrolysats de C. jejuni sont présentés.

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