Methods for producing heterologous disulfide bond-containing...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/70 (2006.01) C07K 14/47 (2006.01) C07K 14/65 (2006.01) C07K 14/81 (2006.01) C12N 9/02 (2006.01) C12N 9/64 (2006.01) C12N 9/72 (2006.01)

Patent

CA 2293611

Disclosed are methods and compositions for producing heterologous disulfide bond containing polypeptides in bacterial cells. In preferred embodiments, the methods involve co-expression of a prokaryotic disulfide isomerase, such as DsbC or DsbG and a gene encoding a recombinant eukaryotic polypeptide. Exemplary polypeptides disclosed include tissue plasminogen activator.

L'invention concerne des procédés et des compositions destinés à la production de polypeptides à ponts disulfure hétérologues dans des cellules bactériennes. Dans des modes de réalisation préférés, les procédés en question font intervenir la co-expression d'une disulfure-isomérase de type procaryote (par exemple DsbC ou DsbG) et d'un gène codant un polypeptide eucaryote de recombinaison. Parmi les exemples de polypeptides donnés figure un activateur du plasminogène tissulaire.

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