Methods for producing libraries of expressible gene sequences

C - Chemistry – Metallurgy – 12 – Q

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Details

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) C12Q 1/06 (2006.01) G01N 33/53 (2006.01) G01N 33/536 (2006.01) G01N 33/543 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2324514

The present invention comprises a method for producing libraries of expressible gene sequences. The method of the invention allows for the simultaneous manipulation of multiple gene sequences and thus allows libraries to be created in an efficient and high throughput manner. The expression vectors containing verified gene sequences can be used to transfect cells for the production of recombinant proteins. The invention further comprises libraries of expressible gene sequences produced using the method of the invention and expression vectors used in the construction of said libraries.

La présente invention concerne une méthode de production de banques de séquences de gènes exprimables. La méthode selon l'invention permet la manipulation simultanée de plusieurs séquences de gènes et permet donc de créer des banques de manière efficace et à un débit élevé. On peut utiliser les vecteurs d'expression afin de produire des protéines de recombinaison. L'invention concerne en outre des banques de séquences de gènes exprimables produites grâce à la méthode selon l'invention et des vecteurs d'expression utilisés dans l'élaboration de ces banques.

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