Methods for producing members of specific binding pairs

Details Methods for producing members of specific binding pairs Methods for producing members of specific binding pairs

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 16/00 (2006.01) C07K 16/18 (2006.01) C07K 16/22 (2006.01) C07K 16/24 (2006.01) C07K 16/28 (2006.01) C07K 16/30 (2006.01) C07K 16/40 (2006.01) C07K 16/42 (2006.01) C07K 16/46 (2006.01) C12N 1/21 (2006.01) C12N 15/10 (2006.01) C12N 15/13 (2006.01) C12N 15/63 (2006.01) G01N 33/531 (2006.01)

Patent

CA 2131151

2131151 9319172 PCTABS00025 Methods, recombinant host cells and kits are disclosed for the production of members of specific binding pairs (sbp), e.g. antibodies, using display on the surface of secreted replicable genetic display packages (rgdps), e.g. filamentous phage. To produce a library of great diversity recombination occurs between first and second vectors comprising nucleic acid encoding first and second polypeptide chains of sbp members respectively, thereby producing recombinant vectors each encoding both a first and a second polypeptide chain component of an sbp member. The recombination may take place in vitro or intracellularly and may be site-specific, e.g. involving use of the l^_o^_x^_P sequence and mutants thereof. Recombination may take place after prior screening or selecting for rgdps displaying sbp members which bind complementary sbp member of interest.

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