Methods for producing micrornas

Details Methods for producing micrornas Methods for producing micrornas

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) A01K 67/02 (2006.01) A61K 31/7088 (2006.01) A61K 48/00 (2006.01) C12N 5/10 (2006.01) C12N 5/16 (2006.01) C12N 15/79 (2006.01) C12N 15/85 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2610265

The invention relates to recombinant vectors for inducible and/or tissue specific expression of double-stranded RNA molecules that interfere with the expression of a target gene. In certain embodiments, the invention relates to the use of Tet (tetracycline)-responsive RNA Polymerase II (Pol II) promoters (e.g., TetON or TetOFF) to direct inducible knockdown in certain cells of an integrated or an endogenous gene, such as p53. The invention also relates to a method for producing transgenic animals (e.g., mice) expressing inducible (such as tetracycline-regulated), reversible, and/or tissue-specific double- stranded RNA molecules that interfere with the expression of a target gene.

L'invention porte sur des vecteurs de recombinaison exprimant des molécules d'ARN double brin inductibles et/ou spécifiques de tissus, interférant avec l'expression d'un gène cible. Dans certaines exécutions l'invention porte sur l'utilisation de promoteurs de l'ARN polymérase répondant à la Tet (tétracycline) (pex, TetON ou TetOFF) et induisant directement dans certaines cellules l'immobilisation d'un gène intégré ou endogène tel que le p53. L'invention porte également sur une méthode d'obtention d'animaux transgéniques (pex des souris) exprimant des molécules d'ARN double brin, inductibles (telles que régulées par la tétracycline), réversibles, et/ou spécifiques de tissus, qui interférent avec l'expression d'un gène cible.

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